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super script vilo cdna synthesis kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher super script vilo cdna synthesis kit
    Super Script Vilo Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/super+script+vilo+cdna+synthesis+kit/high+capacity+cdna+reverse+transcription+kit/pm39742002-101-12-18
    Average 90 stars, based on 1 article reviews
    super script vilo cdna synthesis kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Establishment of a protocol to administer immunosuppressive drugs for iPS cell-derived cardiomyocyte patch transplantation in a rat myocardial infarction model.
    Article Snippet: .. Total RNA was extracted from hiPSCs and hiPSC-CMs after freeze-thawing using the RNeasy Mini Kit (Qiagen, Hilden, Germany) and was reverse transcribed to cDNA using the Super Script VILO cDNA Synthesis kit (Thermo Fisher Scientific, Waltham, MA). .. RT-qPCR was performed using a ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA) using SYBR Green (Applied Biosystems).

    Article Title: Gene-modified mouse expressing human GPC3 polypeptide
    Article Snippet: .. 3 with humans (FIG. 9) were synthesized by reverse transcription reaction using a Super Script VILO cDNA Synthesis Kit (Invitrogen) and using 0.5 μg each of the total RNAs as templates. ..

    Article Title: Ultrasound Stimulation of Piezoelectric Nanocomposite Hydrogels Boosts Chondrogenic Differentiation in Vitro , in Both a Normal and Inflammatory Milieu.
    Article Snippet: The samples were then treated with DNase I (DNA-free kit) and the RNA was quantified using a Nanodrop spectrophotometer (EuroClone S.p.a). .. Reverse transcription was performed using Super Script Vilo cDNA synthesis kit (Life Technologies), according to the manufacturer’s protocol. qRT-PCRwas performed using TBGreen Premix ExTaq (Takara Bio Inc. Shiga 52-0058, Japan) with LightCycler2.0 (Roche Molecular Biochemicals). ..

    cDNA Synthesis:

    Article Title: Establishment of a protocol to administer immunosuppressive drugs for iPS cell-derived cardiomyocyte patch transplantation in a rat myocardial infarction model.
    Article Snippet: .. Total RNA was extracted from hiPSCs and hiPSC-CMs after freeze-thawing using the RNeasy Mini Kit (Qiagen, Hilden, Germany) and was reverse transcribed to cDNA using the Super Script VILO cDNA Synthesis kit (Thermo Fisher Scientific, Waltham, MA). .. RT-qPCR was performed using a ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA) using SYBR Green (Applied Biosystems).

    Article Title: Thymidine Phosphorylase Promotes Abdominal Aortic Aneurysm via VSMC Modulation and Matrix Remodeling in Mice and Humans.
    Article Snippet: Total RNA was isolated from the human aortic vessel wall, frozen AAA samples (AAA #1–#8), healthy donors, or VSMCs using the Qiagen Universal RNA extraction kit. .. One microgram of total RNA was used for cDNA construction using the Super Script VILO cDNA Synthesis Kit (Thermofisher Scientific, Waltham, MA). ..

    Article Title: Anti-tumor agent containing cytarabine, anti- tumor effect enhancing agent which is used in combination with cytarabine, anti-tumor kit, and anti-tumor agent which is used in combination with cytarabine
    Article Snippet: 1.2×106 cells/well of U-937 cells, MOLM-13 cells, HL60 cells, SKM-1 cells, OCI-M2 cells, NOMO-1 cells, MV4-11 cells, THP1 cells were plated in a 6-well plate (Corning Inc., 3506), washed with PBS after 24 hours, and collected. .. RNA was extracted using RNeasy kit (QIAGEN, 74106), and RNA concentration was measured with Nanodrop (Thermo Fisher Scientific, Inc., ND-1000). cDNA was synthesized using the Super Script VILO cDNA Synthesis Kit (Invitrogen, 11754-250) using the extracted RNA as the template according to the specified protocol. ..

    Article Title: RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALS.
    Article Snippet: .. Total RNA was prepared using ISOGEN2 (NIPPON GENE) and was used to generate complementary DNAs (cDNAs) with the Super Script VILO cDNA Synthesis Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Thereafter, 1 μl of primer mix of the target gene and 10 μl of TaqMan universal PCRmaster Mix (Thermo Fisher Scientific) were added to dilute cDNA to 20 μl.

    Article Title: Gene-modified mouse expressing human GPC3 polypeptide
    Article Snippet: .. 3 with humans (FIG. 9) were synthesized by reverse transcription reaction using a Super Script VILO cDNA Synthesis Kit (Invitrogen) and using 0.5 μg each of the total RNAs as templates. ..

    Article Title: Time restricted feeding with or without ketosis influences metabolism-related gene expression in a tissue-specific manner in aged rats
    Article Snippet: RNA was isolated with a cold TRIzol (Thermo Fisher Scientific) extraction, or for samples <30mg, via PureLink RNA Micro Kit (Invitrogen). .. Total RNA was quantified with a NanoDrop UV Visible Spectrophotometer machine. cDNA was synthesized using a Super Script VILO cDNA synthesis kit (Thermo Fisher Scientific) according to the instructions provided. ..

    Article Title: RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALS
    Article Snippet: .. Total RNA was prepared using ISOGEN2 (NIPPON GENE) and was used to generate complementary DNAs (cDNAs) with the Super Script VILO cDNA Synthesis Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Thereafter, 1 μl of primer mix of the target gene and 10 μl of TaqMan universal PCR master Mix (Thermo Fisher Scientific) were added to dilute cDNA to 20 μl.

    Concentration Assay:

    Article Title: Anti-tumor agent containing cytarabine, anti- tumor effect enhancing agent which is used in combination with cytarabine, anti-tumor kit, and anti-tumor agent which is used in combination with cytarabine
    Article Snippet: 1.2×106 cells/well of U-937 cells, MOLM-13 cells, HL60 cells, SKM-1 cells, OCI-M2 cells, NOMO-1 cells, MV4-11 cells, THP1 cells were plated in a 6-well plate (Corning Inc., 3506), washed with PBS after 24 hours, and collected. .. RNA was extracted using RNeasy kit (QIAGEN, 74106), and RNA concentration was measured with Nanodrop (Thermo Fisher Scientific, Inc., ND-1000). cDNA was synthesized using the Super Script VILO cDNA Synthesis Kit (Invitrogen, 11754-250) using the extracted RNA as the template according to the specified protocol. ..

    Synthesized:

    Article Title: Anti-tumor agent containing cytarabine, anti- tumor effect enhancing agent which is used in combination with cytarabine, anti-tumor kit, and anti-tumor agent which is used in combination with cytarabine
    Article Snippet: 1.2×106 cells/well of U-937 cells, MOLM-13 cells, HL60 cells, SKM-1 cells, OCI-M2 cells, NOMO-1 cells, MV4-11 cells, THP1 cells were plated in a 6-well plate (Corning Inc., 3506), washed with PBS after 24 hours, and collected. .. RNA was extracted using RNeasy kit (QIAGEN, 74106), and RNA concentration was measured with Nanodrop (Thermo Fisher Scientific, Inc., ND-1000). cDNA was synthesized using the Super Script VILO cDNA Synthesis Kit (Invitrogen, 11754-250) using the extracted RNA as the template according to the specified protocol. ..

    Article Title: Gene-modified mouse expressing human GPC3 polypeptide
    Article Snippet: .. 3 with humans (FIG. 9) were synthesized by reverse transcription reaction using a Super Script VILO cDNA Synthesis Kit (Invitrogen) and using 0.5 μg each of the total RNAs as templates. ..

    Article Title: Time restricted feeding with or without ketosis influences metabolism-related gene expression in a tissue-specific manner in aged rats
    Article Snippet: RNA was isolated with a cold TRIzol (Thermo Fisher Scientific) extraction, or for samples <30mg, via PureLink RNA Micro Kit (Invitrogen). .. Total RNA was quantified with a NanoDrop UV Visible Spectrophotometer machine. cDNA was synthesized using a Super Script VILO cDNA synthesis kit (Thermo Fisher Scientific) according to the instructions provided. ..

    Spectrophotometry:

    Article Title: Time restricted feeding with or without ketosis influences metabolism-related gene expression in a tissue-specific manner in aged rats
    Article Snippet: RNA was isolated with a cold TRIzol (Thermo Fisher Scientific) extraction, or for samples <30mg, via PureLink RNA Micro Kit (Invitrogen). .. Total RNA was quantified with a NanoDrop UV Visible Spectrophotometer machine. cDNA was synthesized using a Super Script VILO cDNA synthesis kit (Thermo Fisher Scientific) according to the instructions provided. ..



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